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KMID : 0545119950050020110
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 2 p.110 ~ p.113
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Transposon Tn5 Mutagenesis of Bradyrhizobium japonicum
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So Jae-Seong
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Abstract
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Transposon Tn5 was used to induce random insertional mutations in Bradyrhizobium japonicum, a soybean endosymbiont. By genomic Southern blot analysis, transposition events were found to have occurred randomly throughout the B. japonicum genome. After screening 3,626 mutants by auxotrophy test, a histidine auxotroph was isolated. Upon plant infection test, the His^- mutant showed a 3∼4 day delay in nodule formation.
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